Selective permeability of rat liver mitochondria to purified aspartate aminotransferases in vitro
نویسندگان
چکیده
منابع مشابه
Selective permeability of rat liver mitochondria to purified aspartate aminotransferases in vitro.
1. A method was devised to allow determination of intramitochondrial aspartate amino-transferase activity in suspensions of intact mitochondria. 2. Addition of purified rat liver mitochondrial aspartate aminotransferase to suspensions of rat liver mitochondria caused an apparent increase in the intramitochondrial enzyme activity. No increase was observed when the mitochondria were preincubated ...
متن کاملStudies in vitro on amino acid incorporation into purified components of rat liver mitochondria.
Several major protein components of the inner membrane were isolated from rat liver mitochondria after an incubation with (14C)leucine. No significant radioactivity was present in either the fraction containing the oligomycin-sensitive ATPase or that containing cytochromes b and cl. A variable amount of radioactive label was associated with a membranous preparation of cytochrome oxidase. After ...
متن کاملWater permeability of rat liver mitochondria: A biophysical study.
The movement of water accompanying solutes between the cytoplasm and the mitochondrial spaces is central for mitochondrial volume homeostasis, an important function for mitochondrial activities and for preventing the deleterious effects of excess matrix swelling or contraction. While the discovery of aquaporin water channels in the inner mitochondrial membrane provided valuable insights into th...
متن کاملSome Permeability Properties of Isolated Rat Liver Cell Mitochondria
The rates of penetration of various solutes into isolated rat liver mitochondria have been studied. Sodium, potassium, and sucrose were observed to enter the mitochondria until an equilibrium concentration was reached. The diffusion of these solutes, after the first few minutes, followed the predicted diffusion curve for solutes entering a particle with a rate-limiting membrane and instantaneou...
متن کاملPrimary structures of aspartate aminotransferases.
three residues seems to be common to all the serine enzymes and has become known as the ‘charge-relay system’. Its function is to transfer a negative (or partial negative) charge from the aspartic acid residue to the oxygen atom of the hydroxy group of serine. This obviously increases the nucleophilicity of the serine, but the important point is that the charge is developed in the absence of bu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1977
ISSN: 0264-6021
DOI: 10.1042/bj1640685